Author(s): Zahralsadat Hosseini Nasab
The effectiveness of Eucalyptus nanoemulsion as an alternative for chemical control and suppression of destructive pests Meloidogyne javanica was investigated in laboratory conditions. The droplet size of the Eucalyptus nanoemulsion was 23.88 nm and recorded the best results in laboratory research. The uniform structure of eucalyptus nanoemulsion in the liquid produced by this process has improved the reactivity of the particles. The duration of making nanoemulsion was done in the time interval of 0 to 30 minutes in order to investigate the effect of time of nanoemulsion formation and the physical stability of the formulation with this method, the transparency and stability of nanoemulsion will increase with time. The effect of eucalyptus nanoemulsion on the percentage of mortality of the second instar larvae of the root-knot nematode Meloidogyne javanica was investigated in laboratory conditions. The results showed that eucalyptus nanoemulsion can penetrate the cuticle surface and destroy the inner tissue after 24 hours. Also, the results showed that Eucalyptus nanoemulsion has a significant effect on the mortality rate of larvae and the percentage of larval deaths is significantly different in different concentrations of nanoemulsion. In laboratory conditions, using a concentration of 2.76 ppm resulted in the highest loss of second-instar larvae (90%), and the loss rate was 50%, 62%, and 75%, respectively, for concentrations of 2.14, 1.48, and 0.77 ppm it was. Based on the results, a low concentration of nanoemulsion has significant effects on larval mortality, which indicates the nematicidal potential of eucalyptus nanoemulsion.
